Abstract: The Dawangzhuang buried hill zone is a typical representative of Paleozoic oil and gas exploration in the Jiyang Depression, Bohai Bay Basin, and has experienced a complicated accumulation process. Using petrographic observation, microthermometry and abundance of hydrocarbon inclusions, the Paleozoic hydrocarbon charging process in Dawangzhuang buried hill zone was discussed. The study shows that the Paleozoic reservoirs developed hydrocarbon inclusions in the study area. Ordovician hydrocarbon inclusions with pale yellow and blue-green fluorescence distributed along the calcite veins and particle healing joints. Asphalt inclusions and oleaginous are only distributed in Dagu 671 oil reservoir with a small scale. The main peaks of the homogenization temperature are from 80 ℃ to 90 ℃ and from 100 ℃ to 110 ℃; Carboniferous-Permian hydrocarbon inclusions with blue-green fluorescent distributed along the micro-cracks of quartz particles and secondary enlarged edges. The main peak of the homogenization temperature is from 100 ℃ to 110 ℃. Combining with the hydrocarbon generation and expulsion periods of source rocks, it can be concluded that there are two stages of hydrocarbon charging in the Paleozoic in the study area. The first stage of oil and gas charging occurred in the late Dongying period (27~25 Ma), which was a small-scale accumulation of Paleozoic, mainly in the Ordovician; the second stage of oil and gas charging occurred from the end of Guantao period to the end of Minghuazhen period (5~2 Ma), which was a large-scale accumulation of Ordovician and Carboniferous-Permian. The development of asphalt inclusions near the Dagu 671 well area and the fact shown by the Effective Grids containing Oil Inclusions (EGOI) that the ancient oil-water interface is lower than the current one are related to the activity of the Da 1 fault at the end of the Minghuazhen period (2 Ma), resulting in local adjustments and smaller scope of oil and gas reservoir.