Abstract: The Cambrian Longwangmiao Formation in the Sichuan Basin is old, deeply buried with extensive diagenetic transformations, which was affected by important tectonic movements such as Caledonian, Indosinian and Himalayan, so that the hydrocarbon accumulation process is complex. The mineral diagenesis sequence of the Longwangmiao Formation was determined by core observation, petrological type analysis and thin section observation combined with cathodoluminescence characteristics. The characteristics of hydrocarbon fluid inclusions in different diagenetic minerals were analyzed, and the homogenization temperature of symbiotic brine inclusions was tested to clarify reservoirs in the Longwangmiao Formation based on the sequence of mineralization. Combined with the thermal evolution history of the Longwangmiao reservoir in Anyue, the hydrocarbon accumulation periods and time were determined. The results showed that the identified fluid inclusions captured by five diagenetic minerals, such as dolomite and quartz, recorded the five stages of hydrocarbon accumulation events. The first stage is the Middle Triassic filling, the second stage is the Early Jurassic filling, the third stage is the Middle Jurassic wet gas filling, the fourth stage is the late period of Early Cretaceous, and the fifth stage of dry gas filling is the adjustment of the gas reservoir since the Late Cretaceous. The adjustment of gas reservoirs during the Himalayan tectonic movement is an important period for the formation of Longwangmiao Formation gas reservoirs, which is about 40 Ma.