Abstract: The filling and accumulation process of marine oil and gas was studied using oil and gas geochemistry, fluid inclusions and tectonic evolution in Yakela area of northern Tarim Basin. Marine hydrocarbon accumulation took place in Yakela area in two stages:the early (45.5-16.5 Ma) and the middle-late (22-4 Ma) Himalayan, and was featured by multi-stage filling and late accumulation. The late filling and accumulation of marine oil and gas were controlled by the "structural tilting" in Yakela area in the late Himalayan. In the early Himalayan period, the pre-Mesozoic structure was higher in the northeast and lower in the southwest. The range of marine oil and gas filling was wide, and the Yakela and eastern Yakela tectonic traps were favorable in the early Himalayan. In the late Himalayan, the Mesozoic tectonics in Yakela area warped and tilted due to the rapid subsidence of Kuqa Depression. The pre-Mesozoic structure became higher in the southwest and lower in the northeast. The Luntai fault belt and its southern traps are favorable for marine oil and gas filling in the late stage. Meanwhile, the earlier oil and gas reservoirs were damaged or adjusted southwards due to tilting.