Abstract: In the petroleum geology sciences, since the controversy over the theory of oil generation, some inorganic theorists have questioned the criticism of the various sources and defects of the organic origin and advocated an inorganic source. Organic theorists have never systematically responded by geochemistry because the old organic cause theory itself does have some problems. Today, a new theory of oil genesis has matured, namely "oil generation driven by microorganism and carbonate in a waterlogged and closed system". This new theory has made a new upgrade:oil generation by carbonate determinism. This paper was divided into two parts. In the first part, 9 points of skeptical comment were put forward on the theory of petroleum inorganic genesis. The second part briefly explained the role of carbonate synergistic microorganisms in the theory of "oil generation driven by microorganism and carbonate in a waterlogged and closed system". On the theoretical basis, the paper proved that oil could not be generated in any sedimentary basins without ancient carbonate source sediments, defined as "carbonate determinism".