Abstract: In recent years, a number of large biogas fields have been discovered in the Levantine Basin of the Eastern Mediterranean, becoming a new replacement for conventional oil and gas resources. The discovered biogas is mainly pure biogas produced by acetic acid fermentation based on the biochemical characteristics and biogenetic types of biogas. The summary of accumulation conditions and rules showed that the large biogas reservoirs in the study area were controlled by four geologic factors including sufficient gas source, extensive distribution of good sandstones providing efficient migration and charging pathways, constant uplift developing giant anticline traps and a favorable match between reservoir and seal. The belt of continuous uplift provides a favorable location for a giant trap and good reservoir.