Abstract: There are special characteristics in evolution of petroleum system of marine sequences in Chinese complicated superimposited basins under condition of multiple stages of tectonic activity. The development of experimental technology of petroleum geology and geochemistry is playing more and more important roles in construction of basic theory of hydrocarbon accumulation of the superimposited basins and in understand of geologic and geochemical process for petroleum exploration and production. A series of experimental technologies which were suitable for the special and complex superimposited basins were developed in recent years. The most important ones among them included assessment of hydrocarbon generation of source rocks, tracing of hydrocarbon migration and analysis of hydrocarbon accumulation. All of them are very helpful for understanding of hydrocarbon accumulation mechanism and distribution in the superimposited basins.