Abstract: Petroleum inclusions have attracted more and more attention from petroleum geologists due to their special significance in petroleum generation,migration and accumulation.Studies of petroleum inclusion properties are systemic and require combination of various methods,such as microscope fluorescence,Laser Raman Spectroscopy,quantitative grain fluorescence and group inclusion composition analyses.The approaches are helpful in composition analyses of petroleum inclusions and provide reliable evidences for basin analyses,petroleum resource evaluation as well as accumulation stage and migration pathway identification.