Abstract: The study on biomarkers from bitumen and inclusion in reservoir has attracted many petroleum geologists.Since there is no good method to divide the biomarkers from bitumen and inclusions,this study has not been developed much up to present days.This paper selected carbonate reservoir samples from Lunxi thick oil area in the Tarim Basin.Some special methods have been tried to abstract biomar-kers from bitumen and inclusions,and then compared the biomarkers from thick oil.A set of effective method has been established to get biomarkers from bitumen and inclusions.The base line on saturated hydrocarbon chromatogram from thick oil waved seriously and contented abundant 25-norhopane, showing obvious biodegradation.However,the biomarkers from inclusion appear biodegradation phenomenon.This indicates that the forming time of inclusions is earlier than that of effective oil accumulation are different.