Abstract: A method to finely separate saturated hydrocarbon and aromatic hydrocarbon by high performance liquid chromatography(HPLC)was introduced.The oil or bitumen samples were pre prepared by being put in a alumina column,saturated hydrocarbon and aromatic hydrocarbon were eluted together from the column using 90:10 of hexane:ether.After concentrated,the saturated hydrocarbon and aromatic hydrocarbon were ready for further separation by HPLC with the eluent hexane and dichloromethane.The fraction of saturates,monoaromatics,diaromatics and triaromatics would be collected under the control of UV detector and fraction collector.Compared with the method from abroad,the advantages of our method are visible:reagent and time saving.