Abstract: The classification of strata has a great effect on the knowledge about structural evolution, sedimentary environment and even evaluation of hydrocarbon potential in a basin. This paper discussed the characteristics of the Mesozoic stratigraphy in well An'can-1 based on lithology, logging response and seismic reflectance. The framework of the Mesozoic stratigraphic sequence was set up, which is meaningful for the appraisal of hydrocarbon potential in the Hefei Basin.