Abstract: The diagenetic fluid inclusion of reservoirs can reflect the physical and chemical conditions when fluid was trapped and provide important information about hydrocarbon inflow. By studying on fluid inclusion combined with the generation and expulsion history of source rocks, the inflow periods and accumulation process of hydrocarbon in Tuyuke oil field are analyzed in this paper. Research reveals that there are mainly two periods of hydrocarbon inflow process in the field. In which, the Lae Triassic-Early Jurassic is the key accumulation period.